乙酸镍对人支气管上皮细胞系的恶性转化研究

doi:10.3969/j.issn.1004-616x.2003.02.003

癌变·畸变·突变 ›› 2003, Vol. 15 ›› Issue (2): 72-74.doi: 10.3969/j.issn.1004-616x.2003.02.003

乙酸镍对人支气管上皮细胞系的恶性转化研究

陈传德1, 2，吴中亮2，陈家2

1.深圳罗湖区疾病预防控制中心，广东　深圳　518020； 2.广州医学院化学致癌研究所，广东　广州　510182

收稿日期:2002-09-20 修回日期:2002-11-19 出版日期:2003-04-30 发布日期:2003-04-30
通讯作者: 陈家

STUDY ON THE ABILITY OF NICKEL ACETATE TO TRANSFORM 16HBE MALIGNANTLY

CHEN Chuan-de1, 2, WU Zhong-liang2, CHEN Jia-kun2

1. Luohu center for Diease Control and Prevention, Shenzhen 518020; 2. Lnstituke for chemical Carcinogenesis, Guanszhou Medical Colleges Guandzhou 510182, china

Received:2002-09-20 Revised:2002-11-19 Online:2003-04-30 Published:2003-04-30
Contact: CHEN Jia-kun

摘要/Abstract

摘要： 目的：建立乙酸镍(nickel acetate)对永生化人支气管上皮细胞系(16HBE)的恶性转化模型，为下一步关于镍化合物致癌的分子机制的研究提供相应的恶性转化细胞。方法：通过克隆形成率实验确定乙酸镍对16HBE细胞转化浓度后，对16HBE细胞进行分阶段多次染毒：每隔20 d染毒1次，每次染毒24 h。染毒12次后，通过软琼脂集落形成试验和裸鼠体内致瘤试验鉴定细胞恶性转化程度。结果：经乙酸镍多次处理16HBE细胞至75代时，细胞生长速度加快，排列紊乱，失去接触抑制，出现复层生长，并可在软琼脂上生长，且呈剂量反应关系，但在第75代之前的细胞则不能在软琼脂上生长。转化细胞在裸鼠体内成瘤，病理组织学证实为低分化鳞状细胞癌。结论：乙酸镍具有使16HBE细胞发生恶性转化的能力。

关键词: 乙酸镍, 人支气管上皮细胞系, 细胞转化

Abstract: Purpose: To establish malignant transformed human bronchial epithelial cell(16HBE) model by nickel acetate for exploring deeply molecular mechanism of nickel carcinogenesis. Methods: 16HBE cells were treated twelve times(a time every 20 days) with nickel acetate of different concentration in vitro. The features of malignancy of transformed cells were identified by colony forming frequency on soft agar and test of tumorigenesis in nude mice. Results: The 75 th passage cells treated twelve times with nickel acetate proliferated rapidly and exhibited in an extensively random orientation. Compared with that of negative control cell, colony formation efficiency of transformed cells in semisolid agar showed a significant increase and was dose-dependent. But before the 75 th passage, cells had not the ability of growth in semisolid agar. All the transformed cells could formed tumor subcutaneously in the nude mice. The tumors were a poor differentiated squamous cell carcinoma in morphology confirmed by histopathological examination. Conclusion: Nickel sulfide, nickel chloride and nickel acetate all could induce malignant transformation of 16HBE cells.

Key words: nickel acetate, malignant transformation, 16HBE

陈传德, , 吴中亮, 陈家. 乙酸镍对人支气管上皮细胞系的恶性转化研究[J]. 癌变·畸变·突变, 2003, 15(2): 72-74.

CHEN Chuan-de, , WU Zhong-liang, CHEN Jia-kun. STUDY ON THE ABILITY OF NICKEL ACETATE TO TRANSFORM 16HBE MALIGNANTLY[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2003, 15(2): 72-74.

[1]	王熙凯, 孟庆贺, 高燕鲁. miR-223-3p通过调控ECT2诱导非小细胞肺癌细胞凋亡的实验研究[J]. 癌变·畸变·突变, 2021, 33(3): 187-192.
[2]	王丹, 耿兴超, 李波, 王雪, 文海若. 体外细胞转化试验的研究与应用进展[J]. 癌变·畸变·突变, 2018, 30(4): 310-314.
[3]	王颖, 蒲江, 齐乃松, 文海若, 王欣, 胡燕平, 宋捷, 张海洲, 王雪. Bhas 42细胞转化试验高通量检测方法的建立及应用[J]. 癌变·畸变·突变, 2015, 27(4): 288-293.
[4]	庞雅琴 (综述)/ 陈雯 (审校). 体外细胞转化试验研究进展[J]. 癌变·畸变·突变, 2007, 19(6): 511-513.
[5]	王李伟, 仲伟鉴, 应贤平, 周永贵, 周祥凤. 三羟异黄酮对BALB/c-3T3细胞的转化作用[J]. 癌变·畸变·突变, 2005, 17(1): 48-51.
[6]	范保星;张开泰;谢　玲;项小琼;吴德昌. α粒子诱发人支气管上皮细胞恶性转化不同时期差异表达基因cDNA文库的构建[J]. , 2002, 14(1): 1-005.
[7]	楼铁柱,葛世丽;项晓琼;吴德昌. α粒子诱发转化人支气管上皮细胞系BEP2D 中XRCC5 等基因的突变[J]. , 2002, 14(1): 10-012.
[8]	楼铁柱,项晓琼,吴德昌. α粒子诱发BEP2D 细胞转化过程中染色体数目的变化[J]. , 2000, 12(3): 172-173.
[9]	刘家仁1 　庞永王旬1 　张尤恩1 　范　春1 　王贤珍1 　朱振岗1 　E. J . Love2. 用细胞转化实验检测S 江有机污染致癌危险性[J]. , 1999, 11(4): 194-198.
[10]	刘云岗　陈家􏲧 　吴中亮. 结晶型硫化镍诱发BALB/ c - 3T3 细胞恶性转化[J]. , 1999, 11(3): 122-124.

乙酸镍对人支气管上皮细胞系的恶性转化研究

STUDY ON THE ABILITY OF NICKEL ACETATE TO TRANSFORM 16HBE MALIGNANTLY

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics

本文评价